With the third national economic census data in Guangzhou，the R&D personnel，R&D investment，R&D intensity，technology project implementation，institution construction，science and technology achievements and other related activities in Guangzhou scale industrial enterprises in 2013 were analyzed，accompanied by the corresponding countermeasures. The data illustrated that the science and technology input and output in Guangzhou industrial enterprises continued growing，and the innovation ability were strengthened. Those played an important role on the transformation and upgrading of industrial structure and transformation of economic growth in Guangzhou. However，the overall R&D capability in Guangzhou industrial enterprises is not strong，the lack of independent brands and core technologies，as well as highly qualified talents cannot be ignored.